Label-free photoacoustic nanoscopy
نویسندگان
چکیده
منابع مشابه
Label-free photoacoustic nanoscopy.
Super-resolution microscopy techniques - capable of overcoming the diffraction limit of light - have opened new opportunities to explore subcellular structures and dynamics not resolvable in conventional far-field microscopy. However, relying on staining with exogenous fluorescent markers, these techniques can sometimes introduce undesired artifacts to the image, mainly due to large tagging age...
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probability of this quantum receiver did not fall below 10–4 is due to experimental limitations; in particular, the visibility of the interferometer at the beamsplitter used in the displacement operation and the detection efficiency of the singlephoton detector were major sources of imperfection. Imperfect visibility causes errors in photon counts even when a correct displacement operation is c...
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We present label-free functional photoacoustic imaging of the ocular microvasculature in living animals. The anterior segment of an adult mouse was imaged with a laser exposure level well within the American National Standards Institute safety standards. Individual red blood cells traveling along the iris capillaries were clearly resolved, and the hemoglobin oxygen saturation in the iris microv...
متن کاملLabel-free photoacoustic microscopy of peripheral nerves.
Peripheral neuropathy is a common neurological problem that affects millions of people worldwide. Diagnosis and treatment of this condition are often hindered by the difficulties in making objective, noninvasive measurements of nerve fibers. Photoacoustic microscopy (PAM) has the ability to obtain high resolution, specific images of peripheral nerves without exogenous contrast. We demonstrated ...
متن کاملLabel-free photoacoustic microscopy of cytochromes.
Photoacoustic microscopy (PAM) has achieved submicron lateral resolution in showing subcellular structures; however, relatively few endogenous subcellular contrasts have so far been imaged. Given that the hemeprotein, mostly cytochromes in general cells, is optically absorbing around the Soret peak (~420 nm), we implemented label-free PAM of cytochromes in cytoplasm for the first time. By measu...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2014
ISSN: 1083-3668
DOI: 10.1117/1.jbo.19.8.086006